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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
26/08/2014 |
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Data da última atualização: |
05/04/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LEITE, J. P.; BARBOSA, E. G. G.; MARIN, S. R. R.; MARINHO, J. P.; CARVALHO, J. F. C.; PAGLIARINI, R. F.; CRUZ, A. S.; OLIVEIRA, M. C. N.; FARIAS, J. R. B.; NEUMAIER, N.; GUIMARÃES, F. C. M.; YOSHIDA, T.; KANAMORI, N.; FUJITA, Y.; NAKASHIMA, K.; SHINOZAKI, K. Y.; DESIDÉRIO, J. A.; NEPOMUCENO, A. L. |
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Afiliação: |
FCAV UNESP; ELTON GARGIONI G. BARBOSA, CNPQ; SILVANA REGINA ROCKENBACH MARIN, CNPSO; UEL; JOSIRLEY DE FATIMA CORREA CARVALHO, CAPES; FCAV UNESP; ANELISE DA SILVA CRUZ, Mestranda; MARIA CRISTINA NEVES DE OLIVEIRA, CNPSO; JOSE RENATO BOUCAS FARIAS, CNPSO; NORMAN NEUMAIER, CNPSO; FRANCISMAR CORREA MARCELINO GUIMARAES, CNPSO; JIRCAS; JIRCAS; JIRCAS; JIRCAS; JIRCAS; FCAV UNESP; ALEXANDRE LIMA NEPOMUCENO, CNPSo. |
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Título: |
Overexpression of the activated form of the AtAREB1 gene (AtAREB1^QT) improves soybean responses to water deficit. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 13, n. 3, p. 6272-6286, 2014. |
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Idioma: |
Inglês |
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Conteúdo: |
Abscisic acid-responsive element binding protein (AREB1) is a basic domain/leucine zipper transcription factor that binds to the abscisic acid (ABA)-responsive element motif in the promoterWater deficit response in GM soybeans with (AtAREB1^QT) region of ABA-inducible genes. Because AREB1 is not sufficient to direct the expression of downstream genes under non-stress conditions, an activated form of AREB1 (AtAREB1^QT) was created. Several reports claim that plants overexpressing AREB1 or AREB1ΔQT show improveddrought tolerance. In our studies, soybean plants overexpressing (AtAREB1^QT) were characterized molecularly, and the phenotype and drought response of three lines were accessed under greenhouse conditions. Under conditions of water deficit, the transformed plants presented a higher survival rate (100%) than those of their isoline, cultivar BR 16 (40%). Moreover, the transformed plants displayed better water use efficiency and had a higher number of leaves than their isoline. Because the transgenic plants had higher stomatal conductance than its isoline under well-watered conditions, it was suggested that the enhanced drought response of (AtAREB1^QT) soybean plants might not be associated with altered transpiration rates mediated by ABA-dependent stomatal closure. However, it is possible that the smaller leaf area of the transgenic plants reduced their transpiration and water use, causing delayed stressonset. The difference in the degree of wilting and percentage of survival between the 35S-(AtAREB1^QT) and wildtype plants may also be related to the regulation of genes that protect against dehydration because metabolic impairment of photosynthesis, deduced by an increasing internal CO2 concentration, was not observed in the transgenic plants. MenosAbscisic acid-responsive element binding protein (AREB1) is a basic domain/leucine zipper transcription factor that binds to the abscisic acid (ABA)-responsive element motif in the promoterWater deficit response in GM soybeans with (AtAREB1^QT) region of ABA-inducible genes. Because AREB1 is not sufficient to direct the expression of downstream genes under non-stress conditions, an activated form of AREB1 (AtAREB1^QT) was created. Several reports claim that plants overexpressing AREB1 or AREB1ΔQT show improveddrought tolerance. In our studies, soybean plants overexpressing (AtAREB1^QT) were characterized molecularly, and the phenotype and drought response of three lines were accessed under greenhouse conditions. Under conditions of water deficit, the transformed plants presented a higher survival rate (100%) than those of their isoline, cultivar BR 16 (40%). Moreover, the transformed plants displayed better water use efficiency and had a higher number of leaves than their isoline. Because the transgenic plants had higher stomatal conductance than its isoline under well-watered conditions, it was suggested that the enhanced drought response of (AtAREB1^QT) soybean plants might not be associated with altered transpiration rates mediated by ABA-dependent stomatal closure. However, it is possible that the smaller leaf area of the transgenic plants reduced their transpiration and water use, causing delayed stressonset. The difference in the degree of wilting and percentage o... Mostrar Tudo |
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Palavras-Chave: |
Soybean. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/107297/1/maria-c.-n.oliveira.pdf
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Marc: |
LEADER 02774naa a2200337 a 4500
001 1993429
005 2022-04-05
008 2014 bl uuuu u00u1 u #d
100 1 $aLEITE, J. P.
245 $aOverexpression of the activated form of the AtAREB1 gene (AtAREB1^QT) improves soybean responses to water deficit.$h[electronic resource]
260 $c2014
520 $aAbscisic acid-responsive element binding protein (AREB1) is a basic domain/leucine zipper transcription factor that binds to the abscisic acid (ABA)-responsive element motif in the promoterWater deficit response in GM soybeans with (AtAREB1^QT) region of ABA-inducible genes. Because AREB1 is not sufficient to direct the expression of downstream genes under non-stress conditions, an activated form of AREB1 (AtAREB1^QT) was created. Several reports claim that plants overexpressing AREB1 or AREB1ΔQT show improveddrought tolerance. In our studies, soybean plants overexpressing (AtAREB1^QT) were characterized molecularly, and the phenotype and drought response of three lines were accessed under greenhouse conditions. Under conditions of water deficit, the transformed plants presented a higher survival rate (100%) than those of their isoline, cultivar BR 16 (40%). Moreover, the transformed plants displayed better water use efficiency and had a higher number of leaves than their isoline. Because the transgenic plants had higher stomatal conductance than its isoline under well-watered conditions, it was suggested that the enhanced drought response of (AtAREB1^QT) soybean plants might not be associated with altered transpiration rates mediated by ABA-dependent stomatal closure. However, it is possible that the smaller leaf area of the transgenic plants reduced their transpiration and water use, causing delayed stressonset. The difference in the degree of wilting and percentage of survival between the 35S-(AtAREB1^QT) and wildtype plants may also be related to the regulation of genes that protect against dehydration because metabolic impairment of photosynthesis, deduced by an increasing internal CO2 concentration, was not observed in the transgenic plants.
653 $aSoybean
700 1 $aBARBOSA, E. G. G.
700 1 $aMARIN, S. R. R.
700 1 $aMARINHO, J. P.
700 1 $aCARVALHO, J. F. C.
700 1 $aPAGLIARINI, R. F.
700 1 $aCRUZ, A. S.
700 1 $aOLIVEIRA, M. C. N.
700 1 $aFARIAS, J. R. B.
700 1 $aNEUMAIER, N.
700 1 $aGUIMARÃES, F. C. M.
700 1 $aYOSHIDA, T.
700 1 $aKANAMORI, N.
700 1 $aFUJITA, Y.
700 1 $aNAKASHIMA, K.
700 1 $aSHINOZAKI, K. Y.
700 1 $aDESIDÉRIO, J. A.
700 1 $aNEPOMUCENO, A. L.
773 $tGenetics and Molecular Research$gv. 13, n. 3, p. 6272-6286, 2014.
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Embrapa Soja (CNPSO) |
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
09/01/2020 |
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Data da última atualização: |
09/01/2020 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
PRELLWITZ, L.; ARRAIS, A. M.; MELLO, M. R. B. de; VERGANI, G. B.; ESTEVES, S. N.; BARBOSA, N. O.; CARVALHO, B. P.; DIAS, J. H.; OLIVEIRA, M. E. F.; FONSECA, J. F. da. |
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Afiliação: |
LÚCIA PRELLWITZ, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brasil; ALINE MATOS ARRAIS, Universidade Federal Rural do Rio de Janeiro (UFRRJ) - Seropédica, RJ, Brasi; MARCO ROBERTO BOURG DE MELLO, Universidade Federal Rural do Rio de Janeiro (UFRRJ) - Seropédica, RJ, Brasi; GABRIEL BRUN VERGANI, Universidade Estadual Paulista (Unesp) - Jaboticaba, -SP, Brasil; SERGIO NOVITA ESTEVES, CPPSE; NATHALIA OLIVEIRA BARBOSA; BRUNO PENA CARVALHO, CPAF-AC; JENNIFFER HAUSCHILDT DIAS, Universidade Federal de Viçosa (UFV) - Viçosa,MG, Brasil; MARIA EMILIA FRANCO OLIVEIRA, Universidade Estadual Paulista (UNESP) - Jaboticabal-SP, Brasil; JEFERSON FERREIRA DA FONSECA, CNPC. |
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Título: |
Induction of synchronous estrus in Morada Nova ewes: effects of distinct progestagen duration regimen and non-surgical embryo recovery as a tool to access efficiency measurements. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Revista Brasileira de Reprodução Animal, v. 43, n. 2, p. 482, abr./jun. 2019. |
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Idioma: |
Português |
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Notas: |
Edição dos resumos do XXIII Congresso Brasileiro de Reprodução Animal, Gramado, RS, Brasil, 15 a 17 de maio 2019. |
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Conteúdo: |
Abstract: The first stet for applying superovulation is to know the responsiveness of the ewe to different estrous synchronous protocol duration and possible implications on embryo yield. On the other hand for nonsurgical embryo recovery (NSER) a well consolidated protocol should be tested and, in Morada Nova ewes, successful non-surgical uterine access was reported (Fonseca et al., 2019. Reprod Fertil Devel, 31:17-26). In this context, the objective of this study was to evaluate the efficiency different synchronous estrus induction and NSER as additional tool to evaluate the results these protocols in pluriparous Morada Nova ewes. A total of 18 pluriparous sheep were divided into three treatment groups with 6 (T6, n=6), 9 (T9, n=6) and 12 (T12, n=6) days of 60 mg of medroxyprogesterone acetate (Progespon®, Zoetis, Campinas, São Paulo, Brazil). The NSER mean duration was 27.6±1.4 min, with recovery of 99.8% of injected fluid and embryo recovery rate of 62.97% (17/27). These preliminary results indicated that all protocols provided similar results and that NSER can be successfully performed and used as additional toll in synchronous estrous induced Morada Nova ewes independently from the duration of progestogen regimen to assure that viable embryos can be produced by ewes subjected by these protocols.
[Indução do estro sincrônico em ovelhas Morada Nova: efeitos do regime de duração distinto do progestágeno e recuperação não cirúrgica do embrião como uma ferramenta para acessar medidas de eficiência]. MenosAbstract: The first stet for applying superovulation is to know the responsiveness of the ewe to different estrous synchronous protocol duration and possible implications on embryo yield. On the other hand for nonsurgical embryo recovery (NSER) a well consolidated protocol should be tested and, in Morada Nova ewes, successful non-surgical uterine access was reported (Fonseca et al., 2019. Reprod Fertil Devel, 31:17-26). In this context, the objective of this study was to evaluate the efficiency different synchronous estrus induction and NSER as additional tool to evaluate the results these protocols in pluriparous Morada Nova ewes. A total of 18 pluriparous sheep were divided into three treatment groups with 6 (T6, n=6), 9 (T9, n=6) and 12 (T12, n=6) days of 60 mg of medroxyprogesterone acetate (Progespon®, Zoetis, Campinas, São Paulo, Brazil). The NSER mean duration was 27.6±1.4 min, with recovery of 99.8% of injected fluid and embryo recovery rate of 62.97% (17/27). These preliminary results indicated that all protocols provided similar results and that NSER can be successfully performed and used as additional toll in synchronous estrous induced Morada Nova ewes independently from the duration of progestogen regimen to assure that viable embryos can be produced by ewes subjected by these protocols.
[Indução do estro sincrônico em ovelhas Morada Nova: efeitos do regime de duração distinto do progestágeno e recuperação não cirúrgica do embrião como uma ferramenta para ace... Mostrar Tudo |
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Thesagro: |
Ovelha; Ovino; Progesterona; Reprodução Animal; Superovulação; Transferência de Embrião. |
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Thesaurus NAL: |
Embryo transfer; Progesterone; Sheep; Small ruminants; Superovulation. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02784nam a2200361 a 4500
001 2118451
005 2020-01-09
008 2019 bl uuuu u01u1 u #d
100 1 $aPRELLWITZ, L.
245 $aInduction of synchronous estrus in Morada Nova ewes$beffects of distinct progestagen duration regimen and non-surgical embryo recovery as a tool to access efficiency measurements.$h[electronic resource]
260 $aRevista Brasileira de Reprodução Animal, v. 43, n. 2, p. 482, abr./jun. 2019.$c2019
500 $aEdição dos resumos do XXIII Congresso Brasileiro de Reprodução Animal, Gramado, RS, Brasil, 15 a 17 de maio 2019.
520 $aAbstract: The first stet for applying superovulation is to know the responsiveness of the ewe to different estrous synchronous protocol duration and possible implications on embryo yield. On the other hand for nonsurgical embryo recovery (NSER) a well consolidated protocol should be tested and, in Morada Nova ewes, successful non-surgical uterine access was reported (Fonseca et al., 2019. Reprod Fertil Devel, 31:17-26). In this context, the objective of this study was to evaluate the efficiency different synchronous estrus induction and NSER as additional tool to evaluate the results these protocols in pluriparous Morada Nova ewes. A total of 18 pluriparous sheep were divided into three treatment groups with 6 (T6, n=6), 9 (T9, n=6) and 12 (T12, n=6) days of 60 mg of medroxyprogesterone acetate (Progespon®, Zoetis, Campinas, São Paulo, Brazil). The NSER mean duration was 27.6±1.4 min, with recovery of 99.8% of injected fluid and embryo recovery rate of 62.97% (17/27). These preliminary results indicated that all protocols provided similar results and that NSER can be successfully performed and used as additional toll in synchronous estrous induced Morada Nova ewes independently from the duration of progestogen regimen to assure that viable embryos can be produced by ewes subjected by these protocols. [Indução do estro sincrônico em ovelhas Morada Nova: efeitos do regime de duração distinto do progestágeno e recuperação não cirúrgica do embrião como uma ferramenta para acessar medidas de eficiência].
650 $aEmbryo transfer
650 $aProgesterone
650 $aSheep
650 $aSmall ruminants
650 $aSuperovulation
650 $aOvelha
650 $aOvino
650 $aProgesterona
650 $aReprodução Animal
650 $aSuperovulação
650 $aTransferência de Embrião
700 1 $aARRAIS, A. M.
700 1 $aMELLO, M. R. B. de
700 1 $aVERGANI, G. B.
700 1 $aESTEVES, S. N.
700 1 $aBARBOSA, N. O.
700 1 $aCARVALHO, B. P.
700 1 $aDIAS, J. H.
700 1 $aOLIVEIRA, M. E. F.
700 1 $aFONSECA, J. F. da
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